| 货号 | 规格 | 价格 |
|---|---|---|
| FL167 | ||
| 10μg | ¥198.00 | |
| 100μg | ¥838.00 | |
| 1mg | ¥2980.00 | |
| 10mg | ¥17800.00 |
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| Appearance | Sterile colorless liquid. |
|---|---|
| Purity | > 98% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.01 EU/μg of protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Tag | Tag free. |
| Activity | Fully biologically active when compared to standard. 1mg of recombinant benzonase contains activity equivalent to at least 1.5×10^6 units. |
| Formulation | A 0.2 μm filtered concentrated solution in 50 mM Tris, with 20 mM NaCl, 2 mM MgCl2, 50% (v/v) Glycerol, pH 8.0. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P13717 Ala22-Asn266 |
|---|---|
| Amino acid sequence | ADTLESIDNCAVGCPTGGSSNVSIVRHAYTLNNNSTTKFANWVAYHITKDTPASGKTRNWKTDPALNPADTLAPADYTGANAALKVDRGHQAPLASLAGVSDWESLNYLSNITPQKSDLNQGAWARLEDQERKLIDRADISSVYTVTGPLYERDMGKLPGTQKAHTIPSAYWKVIFINNSPAVNHYAAFLFDQNTPKGADFCQFRVTVDEIEKRTGLIIWAGLPDDVQASLKSKPGVLPELMGCKN |
| Molecular weight | Approximately 26.8 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids. |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 6 to 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Benzonase is for research use only and not for use in diagnostic or therapeutic procedures. |
| Benzonase is a nuclease that Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3' position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA. Recombinant benzonase is a 26.8 kDa nuclease consisting of 246 amino acid. |
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