| 货号 | 规格 | 价格 |
|---|---|---|
| FL011 | ||
| 10μg | ¥328.00 | |
| 50μg | ¥998.00 | |
| 100μg | ¥1880.00 | |
| 500μg | ¥9180.00 | |
| 1mg | ¥15800.00 |
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| Appearance | Sterile filtered White lyophilized (freeze-dried) powder. |
|---|---|
| Purity | > 97% by SDS-PAGE or HPLC. |
| Endotoxin | < 0.01 EU/μg of rApi m1 protein as determined by LAL method. |
| Expression System | Expressed in E. coli. |
| Tag | Tag free. |
| Activity | Strong Reactivity to honeybee stung positive serum by IGEs test. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Accession # | P00630 Ile34-Tyr167 |
|---|---|
| Amino acid sequence | IIYPGTLWCGHGNKSSGPNELGRFKHTDACCRTHDMCPDVMSAGESKHGLTNTASHTRLSCDCDDKFYDCLKNSADTISSYFVGKMYFNLIDTKCYKLEHPVTGCGERTEGRCLHYTVDKSKPKVYQWFDLRKY |
| Molecular weight | Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids. |
| Synonyms | PLA2 |
| Stability & Storage | Shipped on wet ice. For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. |
| Precautions | Recombinant Apis Mellifera Phospholipase A2/Api m1 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Api m1, a glycoprotein of the phospholipase 2 (PLA2) family, is a major allergen of Apis mellifera (honeybee) venom (HBV) and a marker allergen for genuine sensitization to this venom. Api m 1 contributes 12-16% of venom dry weight. Api m 1 is consistently found as a major allergen in HBV-allergic patients. Of the 12 known allergens within HBV, Api m 1 is considered the most prominent allergen, in terms of prevalence of sensitization, levels of specific IgE to Api m 1, and quantitative correlation between Api m 1 and HBV specific IgE. Api m 1 does not cross-react with phospholipases of the PLA1 family found in Vespid venoms, therefore, Api m 1 is a marker allergen allowing to discriminate between HBV and Vespid venom sensitization. Recombinant Api m1 is a 15.2kDa globular protein containing 134 amino acid residues. |
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