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Anti-Collagen I Rabbit mAb [29E23D18]

Purified Rabbit Recombinant Monoclonal Antibody

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名称: Anti-Collagen I Rabbit mAb [29E23D18] 货号: R013724 应用: IHC-P, ELISA, IF (Tissue) 稀释比: IHC-P 1:200; IF 1:100 种属反应性: Human 规格: 100µL; 50µL; 20µL 价格: ¥1780.00; ¥1080.00; ¥588.00 链接: http://www.epizyme.cn/pro_show_kt.aspx?productid=24891
货号 规格 价格
R013724
100µL ¥1780.00
50µL ¥1080.00
20µL ¥588.00
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Immunohistochemistry - Anti-Collagen I Rabbit mAb [29E23D18]

Sample: Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013724 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Collagen I Rabbit mAb [29E23D18]

Sample: Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013724 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Collagen I Rabbit mAb [29E23D18]

Sample: Paraformaldehyde-fixed, paraffin embedded human beast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013724 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Collagen I Rabbit mAb [29E23D18]

Sample: Paraformaldehyde-fixed, paraffin embedded human beast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013724 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-Collagen I Rabbit mAb [29E23D18]

Sample: Paraformaldehyde-fixed, paraffin embedded human colorectal carcinoma tissue

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013724 at 1:100 dilution

Secondary antibody: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Product Name Anti-Collagen I Rabbit mAb
Description Purified Rabbit Recombinant Monoclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
 IHC-P, ELISA, IF (Tissue)
Dilution IHC-P 1:200; IF 1:100
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone No. 29E23D18
Isotype IgG
Target / Specificity A synthesized peptide derived from human Collagen I
Format Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.
Gene ID 1277
Synonyms OI1, OI2, OI3, OI4, EDSC.
Primary Accession P02452
Storage Shipped at 4℃. Upon delivery aliquot. Store at 4℃ short term (1~2 weeks). Store at -20℃ for 2 years. Avoid freeze / thaw cycles.
Precautions Anti-Collagen I Rabbit mAb [29E23D18] is for research use only and not for use in diagnostic or therapeutic procedures.
Background This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

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