无需浪费珍贵的样品重新制胶电泳来检测不同的一抗，只需使用本抗体剥离缓冲液简单地处理膜，即可除去第一种一抗。通常此步骤只需10~20min，耗时多少依赖于膜上一抗的结合量。在抗体剥离之后，即可使用一种新的一抗进行重新检测。
节省样品
同一张膜的相同样品可用于多种检测省时
不需重新制胶电泳高效快速
去除效果好，远优于自制缓冲液温和配方
对转印膜上结合的靶蛋白损伤极微无气味
无刺鼻气味，更环保完成Western Blot发光检测后，用TBS/T或PBS/T漂洗膜5min；
加入10~20mL剥离液覆盖膜。在摇床上漂洗10~20min。剥离时间视膜上抗体结合量的多少而定；
倒掉剥离液，加入TBS/T或PBS/T漂洗膜5min。此时可在膜上滴上显色发光液以快速检验抗体剥离效果；
用合适的封闭液重新封闭膜，即可再次杂交其它抗体；
使用本剥离液，膜可进行至少10次以上剥离而不会显著影响信号强度。以GAPDH为例子的实验显示，连续剥离6h，即相当于剥离24次(以每次15min计)后，GAPDH的信号没有显著降低。
1. 本产品不建议重复使用；
2. 转印膜接触剥离液会呈透明状，此为正常现象，请放心使用；
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
4. 本产品仅限科研使用。
Liu, C., Xu, Y., Yang, G., Tao, Y., Chang, J., Wang, S., ... & Zeng, Y. Niche Inflammatory Signal Regulates Periodical Mammary Regeneration and Safeguards Stem Cell Survival Under Cytotoxic Stress. Available at SSRN 4431123.(IF 25.269)
Liu, C., Xu, Y., Yang, G., Tao, Y., Chang, J., Wang, S., ... & Zeng, Y. A. (2024). Niche inflammatory signals control oscillating mammary regeneration and protect stem cells from cytotoxic stress. Cell Stem Cell, 31(1), 89-105.(IF 23.9)
Hao K, Chen Y, Yan X, et al. Cilia locally synthesize proteins to sustain their ultrastructure and functions[J]. Nature communications, 2021, 12(1): 1-16.(IF 17.694)
Ma, J. C., Luo, T., Feng, B., Huang, Z., Zhang, Y., Huang, H., ... & Cui, Z. K. (2023). Exploring the translational potential of PLGA nanoparticles for intra-articular rapamycin delivery in osteoarthritis therapy. Journal of Nanobiotechnology, 21(1), 361.(IF 10.2)
Liu, F., Zhang, X., Peng, Y., Zhang, L., Yu, Y., Hua, P., ... & Zhang, L. (2021). miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3. Cell Reports, 35(10), 109225.(IF 9.423)
Li, W., Zhuang, T., Wang, Z., Wang, X., Liu, L., Luo, Y., ... & Ding, L. (2023). Red ginseng extracts ameliorate high-fat diet-induced obesity and insulin resistance by activating the intestinal TGR5-mediated bile acids signaling pathway. Phytomedicine, 119, 154982.(IF 7.9)
Zhang, F., Lin, B., Huang, S., Wu, P., Zhou, M., Zhao, J., ... & Huang, D. (2023). Melatonin Alleviates Retinal Ischemia–Reperfusion Injury by Inhibiting p53–Mediated Ferroptosis. Antioxidants, 12(6), 1173.(IF 7.675)
Gong, F., Ma, J. C., Jia, J., Li, F. Z., Wu, J. L., Wang, S., ... & Cui, Z. K. (2021). Synergistic effect of the anti-PD-1 antibody with blood stable and reduction sensitive curcumin micelles on colon cancer. Drug Delivery, 28(1), 930-942.(IF 6.419)
Liu, Z., Wen, J., Wu, C., Hu, C., Wang, J., Bao, Q., ... & Shen, Y. (2020). MicroRNA-200a induces immunosuppression by promoting PTEN-mediated PD-L1 upregulation in osteosarcoma. Aging (Albany NY), 12(2), 1213.(IF 4.831)
Copyright © 2023上海雅酶生物医药科技有限公司（Epizyme Biotech）
沪ICP备2021002422号
