增强型皮克级化学发光液是一种采用新型配方的辣根过氧化物酶(HRP)底物化学发光液，具有灵敏度极高、性能稳定、半衰期长、背景(本底)极低、重复性好等优点。它可以用于固相膜(如 NC、PVDF)上结合的蛋白(Western Blot)或核酸(Southern/Northern Blot)的检测，既可感光X光胶片，也适用于荧光CCD相机的荧光扫描。
高灵敏度
可检测低至1pg~100fg的抗原，适用于痕量抗原的检测本底极低
即使长时间曝光，背景仍然极低，远远优于国产与进口同类产品半衰期长
信号衰减慢，6h以上仍可感光X光胶片稳定性好
溶液可在室温避光保存12个月转膜及抗体结合。采用常规方法进行转膜、封闭、一抗、HRP-二抗结合，直至二抗洗涤完毕；
等体积混匀P-ECL A和P-ECL B，配制化学发光工作液（每平方厘米的转印膜约需0.1mL的工作液）；
将化学发光液均匀滴到膜上，反应约3min。如要检测的蛋白信号很高时，请尽量不要让化学发光工作液处于静止状态，可将加了工作液的膜置于摇床上混动约3min，以避免局部的反应物过度消耗，影响检测信号的线性范围；
取出膜，沥去多余的发光工作液，将膜包裹于透明的保护夹中；
X光片曝光：在暗室中将膜对X光片曝光。曝光时间从几秒到几分钟不等。将底片显影，如有必要，可根据显影结果调整曝光时间进行第二次曝光；
CCD成像仪：无需将膜置于保护夹中，可直接用于拍照。拍照按仪器使用说明进行；
使用过的膜可剥离抗体后进行多次杂交。
1. P-ECL A及发光工作液应避免强光照射而导致灵敏度降低；
2. 增强型皮克级化学发光液灵敏度很高，因此应避免由于使用的抗体浓度过高而造成背景升高现象；
3. NaN3会抑制HRP的活性，因此二抗的保存或回收应避免使用NaN3；
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
5. 本产品仅限科研使用。
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