Omni-ECLTM基础型化学发光检测试剂盒(皮克级)是一款非放射性的化学发光系统，可用于检测结合到膜上的蛋白所偶联的辣根过氧化物酶(HRP)的活性。本产品包括增强的鲁米诺底物及稳定的过氧化物体系，能检测低至皮克级的微量蛋白，适用于常规Western Blot的化学发光。
高灵敏度
快速检测低至皮克量的蛋白，性能明显优于同级别其它高端品牌产品高性价比
与其它同级别化学发光底物相比，性价比极高从洗涤缓冲液中取出转印膜，沥去多余的液体，使膜保持湿润状态；
等体积混匀PicoLight ECL Substrate和Oxidant，配制化学发光工作液(每平方厘米的转印膜约需0.1mL的工作液)；
将化学发光工作液均匀滴到膜上，反应1~2min。如要检测的蛋白信号很高，请尽量不要让化学发光工作液处于静止状态，可将加了工作液的膜置于摇床上混动1~2min，以避免局部的反应物过度消耗，影响检测信号的线性范围；
取出膜，沥去多余的发光工作液，将膜包裹于透明的保护夹中；
X光片曝光：在暗室中将膜对X光片曝光。曝光时间从几秒到几分钟不等。将底片显影，如有必要，可根据显影结果调整曝光时间进行第二次曝光；
CCD成像仪：无需将膜置于保护夹中，可直接用于拍照。拍照按仪器使用说明进行；
使用过的膜可剥离抗体后进行多次杂交。
1. PicoLight ECL Substrate及发光工作液应避免强光照射而导致灵敏度降低；
2. Omni-ECLTM发光液灵敏度很高，因此应避免由于使用的抗体浓度过高而造成背景升高现象；
3. NaN3会抑制HRP的活性，因此二抗的保存或回收应避免使用NaN3；
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
5. 本产品仅限科研使用。
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