GAPDH Rabbit Polyclonal Antibody
本抗体为兔多克隆抗体。GAPDH(甘油醛-3-磷酸脱氢酶)是参与糖酵解的一种关键酶,由 4个36kDa的亚基组成。除参与糖酵解外,有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程,包括膜融合(membrane fusion)、微管成束(microtubule bundling)、磷酸转移酶(phosphotransferase)激活、核内RNA出核、DNA复制与DNA修复等。GAPDH蛋白几乎在所有组织中都高水平表达,常用于Western Blot检测中校正系统的内参。
缓冲液
10mM PBS(pH7.4),50% glycerol
防腐剂
0.02% sodium azide
应用种属
人、大鼠、小鼠、猴、鸡、仓鼠、绵羊、非洲爪蟾
应用范围
Western Blot(WB)、Inmunohistochemistry(IHC)
推荐浓度(最适浓度需根据具体情况调试)
Western Blot:1:3,000
Inmunohistochemistry:1:200
1. 为了获得实验最佳效果,请根据实验调整最佳工作稀释度;
2. 为了您的安全和健康,请穿实验服并戴一次性手套操作;
3. 本产品仅限科研使用。
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