PVDF膜即聚偏二氟乙烯膜（polyvinylidene fluoride）是蛋白质印迹法中常用的一种固相支持物。PVDF膜是疏水性的，膜孔径有大有小，随着膜孔径的不断减小，膜对低分子量的蛋白结合就越牢固。大于20KD的蛋白选用0.45μm的膜，小于20KD的蛋白选用0.22μm的膜。PVDF膜在使用时需预处理，用Methanol处理的目的是活化膜上的正电基团，使其更容易与带负电的蛋白结合。PVDF膜具有较高的机械强度，是印迹法中的理想固相支持物材料。
1. 机械强度与坚韧度高；
2. 防霉菌性；
3. 高耐磨性；
4. 对气体和液体的高耐渗透性；
5. 耐热稳定性好；
6. 阻燃，低烟。
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