WJ101由15kDa~150kDa范围的9个蛋白条带组成(15kDa、20kDa、25kDa、35kDa、40kDa、50kDa、70kDa、100kDa、150kDa)。WJ102、WJ103和WJ106由10kDa~250kDa范围的11个蛋白条带组成(10kDa、15kDa、20kDa、25kDa、35kDa、40kDa、50kDa、70kDa、100kDa、150kDa、250kDa)。由低到高的分子量范围可监测电泳过程中的蛋白分离情况、判断目标蛋白的分子量，并且可以监测Western Blot实验中的转膜效率。25kDa、70kDa两条带结合有红色或绿色染料，更便于实时观察电泳进程、判断蛋白分子量，其中WJ106特别适用于近红外荧光成像系统。本品推荐上样量为5μL。
缓冲液成分：62.5mM Tris-HCl(pH7.5/25℃)，1mM EDTA，2%(W/V)SDS，10mM DTT，33%(W/V)glycerol。
| WJ101(15 kDa~150 kDa)250 μL×2 | WJ101L(15 kDa~150 kDa)250 μL×10 |
| WJ102(10 kDa~250 kDa)250 μL×2 | WJ102L(10 kDa~250 kDa)250 μL×10 |
| WJ103(10 kDa~250 kDa)250 μL×2 | WJ103L(10 kDa~250 kDa)250 μL×10 |
| WJ106(10 kDa~250 kDa)250 μL×2 | WJ106L(10 kDa~250 kDa)250 μL×10 |
| 注：WJ106 系列适用于近红外荧光成像系统。 | |
高准确性
蛋白条带分子量相较于同级别其它高端品牌产品更准确分布合理
蛋白条带分布更合理低杂交反应
条带蛋白去除蛋白质标签，最大程度降低抗体杂交反应高性价比
与其它同级别产品相比，性价比相当高
加样前在室温放置数分钟，解冻后，轻轻摇匀，以确保溶液混合均匀；
取用适量体积加样于凝胶孔，推荐用量如下：
0.75~1.0mm凝胶，上样5μL/孔；1.5mm凝胶，上样10μL/孔。也可根据情况适当减少加样量。
取用结束，盖上管盖，放回4℃或-20℃。
注：以上样5μL为例，WJ101、WJ102、WJ103及WJ106每个条带蛋白含量约为1μg~3μg。
不同电泳缓冲条件下，WJ101、WJ102、WJ103 及 WJ106 各条带指示分子量(kDa)
1. 本品已包含上样缓冲液，直接使用，不可加热煮沸；
2. 为避免反复冻融及污染，可将本产品分装后，-20℃保存；
3. 预染蛋白质分子量标准仅用于分子量的近似参考，批次间差异约为5%；
4. 若使用近红外荧光成像系统，请选用WJ106；
5. 在低浓度凝胶中，低分子量条带将会与前沿共迁移而无法分出；
6. 在不同凝胶和缓冲液系统中，预染蛋白质的迁移率会有所差别，可用非预染蛋白质分子量标准进行校正，并按校正后的结果正常使用；
7. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
8. 本产品仅限科研使用。
Zeng, Y., Wu, R., Wang, F., Li, S., Li, L., Li, Y., ... & Gong, S. (2023). Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice. Cell Host & Microbe, 31(5), 766-780.(IF 31.316)
Ren, L., Gao, Y., & Cheng, Y. (2022). A manganese (II)-based coordinative dendrimer with robust efficiency in intracellular peptide delivery. Bioactive materials, 9, 44-53.(IF 16.874)
Cui, Y., Hong, S., Xia, Y., Li, X., He, X., Hu, X., ... & Mao, L. (2023). Melatonin Engineering M2 Macrophage‐Derived Exosomes Mediate Endoplasmic Reticulum Stress and Immune Reprogramming for Periodontitis Therapy. Advanced Science, 2302029.(IF 15.1)
Li, M., Li, G., Yang, Y., Zong, J., Fu, X., Htet, A. L. H., ... & Yu, T. (2023). piRNA-823 is a novel potential therapeutic target in aortic dissection. Pharmacological Research, 196, 106932.(IF 9.3)
Chen, Y., Zhai, M. J., Mehwish, N., Xu, M. D., Wang, Y., Gong, Y. X., ... & Lee, B. H. (2022). Comparison of globular albumin methacryloyl and random-coil gelatin methacryloyl: Preparation, hydrogel properties, cell behaviors, and mineralization. International Journal of Biological Macromolecules, 204, 692-708.(IF 8.025)
Shi, D., Si, Z., Xu, Z., Cheng, Y., Lin, Q., Fu, Z., ... & Cheng, D. (2022). Synthesis and Evaluation of 68Ga-NOTA-COG1410 Targeting to TREM2 of TAMs as a Specific PET Probe for Digestive Tumor Diagnosis. Analytical Chemistry, 94(9), 3819-3830.(IF 8.008)
Wang, J., Liao, H., Ban, J., Li, S., **ong, X., He, Q., ... & Liu, L. (2023). Multifunctional Near-Infrared Dye IR-817 Encapsulated in Albumin Nanoparticles for Enhanced Imaging and Photothermal Therapy in Melanoma. International Journal of Nanomedicine, 4949-4967.(IF 8)
Yan, B., Jiao, X., Zhu, H., Wang, Q., Huang, J., Zhao, J., ... & Zhang, H. (2020). Chemical interactions involved in microwave heat-induced surimi gel fortified with fish oil and its formation mechanism. Food Hydrocolloids, 105, 105779.(IF 7.053)
Wang, J., Jia, J., He, Q., Xu, Y., Liao, H., Xiong, X., ... & Sun, C. (2023). A novel multifunctional mitochondrion-targeting NIR fluorophore probe inhibits tumour proliferation and metastasis through the PPARγ/ROS/β-catenin pathway. European Journal of Medicinal Chemistry, 115435.(IF 6.7)
Fan, X., Zhang, Y., Song, Y., Zhao, Y., Xu, Y., Guo, F., ... & Qin, G. (2023). Compound Danshen Dripping Pills Moderate Intestinal Flora and the TLR4/MyD88/NF-κB Signaling Pathway in Alleviating Cognitive Dysfunction in Type 2 Diabetic KK-Ay Mice. Phytomedicine, 154656.(IF 6.656)
Zhou, L. M., Fan, J. H., Xu, M. M., Xiong, M. Y., Wang, Q. J., Chai, X., ... & Ye, X. L. (2023). Epiberberine regulates lipid synthesis through SHP (NR0B2) to improve non-alcoholic steatohepatitis. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 166639.(IF 6.633)
Peng, M., Lu, D., Liu, J., Jiang, B., & Chen, J. (2021). Effect of roasting on the antioxidant activity, phenolic composition, and nutritional quality of pumpkin (Cucurbita pepo L.) seeds. Frontiers in Nutrition, 8, 647354.(IF 6.576)
Liu, Q., Zhao, S., Meng, F., Wang, H., Sun, L., Li, G., ... & Chen, F. (2021). Nrf2 Down-Regulation by Camptothecin Favors Inhibiting Invasion, Metastasis and Angiogenesis in Hepatocellular Carcinoma. Frontiers in Oncology, 11, 661157.(IF 6.244)
Li, S., Zhu, H., He, Y., Hong, N., Wang, G., & Wang, L. (2023). BdCV1-Encoded P3 Silencing Suppressor Identification and Its Roles in Botryosphaeria dothidea, Causing Pear Ring Rot Disease. Cells, 12(19), 2386.(IF 6)
Geng, C., Liu, S., Wang, J., Wang, S., Zhang, W., Rong, H., ... & Zhang, Y. (2023). Targeting the cochlin/SFRP1/CaMKII axis in the ocular posterior pole prevents the progression of nonpathologic myopia. Communications Biology, 6(1), 884.(IF 5.9)
Sun, H., Wang, S., Yang, Z., Tian, L., Li, X., Zhou, J., & Wang, B. (2023). Chordin like-1 regulates osteoblast and adipocyte differentiation through stabilizing insulin-like growth factor binding protein 3. Stem Cells, sxad009.(IF 5.845)
Huang, Z., Su, Q., Li, W., Ren, H., Huang, H., & Wang, A. (2021). Suppressed mitochondrial respiration via NOX5-mediated redox imbalance contributes to the antitumor activity of anlotinib in oral squamous cell carcinoma. Journal of Genetics and Genomics, 48(7), 582-594.(IF 5.723)
Liu, J., Lin, W. P., Su, W., Wu, Z. Z., Yang, Q. C., Wang, S., ... & Sun, Z. J. (2023). Sunitinib attenuates reactive MDSCs enhancing anti-tumor immunity in HNSCC. International Immunopharmacology, 119, 110243.(IF 5.714)
Tang, A. L., Liu, X. Y., Gao, N., Hu, T. P., Yan, S. T., & Zhang, G. Q. (2023). Dl-3-n-butylphthalide improves intestinal microcirculation disorders in septic rats by regulating the PI3K/AKT signaling pathway and autophagy. International Immunopharmacology, 118, 110049.(IF 5.714)
Fu, X. Z., & Wang, Y. (2023). Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway. Molecular Medicine, 29(1), 95.(IF 5.7)
Shi, D., Xu, Z., Cheng, Y., Lin, Q., Si, Z., Fu, W., ... & Cheng, D. (2023). 124I-Labeled Immuno-PET Targeting hTREM2 for the Diagnosis of Gastric Carcinoma. Molecular Pharmaceutics, 20(4), 2235-2244.(IF 5.364)
Zhu, Q., Tang, X., Lu, M., & Chen, J. (2022). Structure and immunoreactivity of purified Siberian apricot (Prunus sibirica L.) kernel allergen under high hydrostatic pressure treatment. Food Bioscience, 48, 101727.(IF 5.318)
Zhong, Y., Liu, J., Cheng, X., Zhang, H., Zhang, C., Xia, Z., ... & Jiang, F. (2021). Design, synthesis and biological evaluations of diverse Michael acceptor-based phenazine hybrid molecules as TrxR1 inhibitors. Bioorganic Chemistry, 109, 104736.(IF 5.275)
Song, J., Tang, Y., & Song, F. (2023). Lnc‑RGS5 sponges miR‑542‑5p to promote FoxM1/VEGFA signaling and breast cancer cell proliferation. International Journal of Oncology, 63(4), 1-13.(IF 5.2)
Li, K., Yu, S., Yu, D., Lin, H., Liu, N., & Wu, A. (2022). Biodegradation of Fumonisins by the Consecutive Action of a Fusion Enzyme. Toxins, 14(4), 266.(IF 5.075)
Zhao, S., Shi, D., Su, C., Jiang, W., Zhang, C., Liang, T., & Hou, G. (2020). IL-27Rα: a novel molecular imaging marker for allograft rejection. International Journal of Molecular Sciences, 21(4), 1315.(IF 4.556)
Cai, Y., Li, X., Pan, Z., Zhu, Y., Tuo, J., Meng, Q., ... & Pan, Y. (2020). Anthocyanin ameliorates hypoxia and ischemia induced inflammation and apoptosis by increasing autophagic flux in SH-SY5Y cells. European Journal of Pharmacology, 883, 173360.(IF 4.432)
Wu, X., Liu, C., Wang, J., Guan, Y., Song, L., Chen, R., & Gong, M. (2022). Catalpol Exerts Antidepressant-Like Effects by Enhancing Anti-oxidation and Neurotrophy and Inhibiting Neuroinflammation via Activation of HO-1. Neurochemical Research, 1-17.(IF 4.414)
Bao, L., Chu, Y., & Kang, H. (2023). SNAI1-activated long non-coding RNA LINC01711 promotes hepatic fibrosis cell proliferation and migration by regulating XYLT1. Genomics, 115(3), 110597.(IF 4.31)
Xie, Y., Jiang, W., Cao, J., & Xie, H. (2021). Dexmedetomidine attenuates acute kidney injury in children undergoing congenital heart surgery with cardiopulmonary bypass by inhibiting the TLR3/NF-κB signaling pathway. American Journal of Translational Research, 13(4), 2763.(IF 4.06)
Shang, S., He, Z., Hou, W., Chen, X., Zhao, X., Han, H., ... & Tai, F. (2023). Molecular cloning, expression analysis and functional characterization of chicken cytochrome P450 27A1: A novel mitochondrial vitamin D3 25-hydroxylase. Poultry Science, 102(8), 102747.(IF 4.014)
Tang, T., Zhu, Z., He, Z., Wang, F., Chen, H., Liu, S., ... & Liu, S. (2023). DLX5 regulates the osteogenic differentiation of spinal ligaments cells derived from ossification of the posterior longitudinal ligament patients via NOTCH signaling. JOR Spine, e1247.(IF 3.757)
Fan, J., Wang, Y., Liang, X., Peng, Y., Li, S., Li, X., ... & Li, Y. (2023). B7-H6 enhances F-actin rearrangement by targeting c-MYC activation to promote medulloblastoma migration and invasion. Medical Oncology, 40(3), 85.(IF 3.738)
Shi, D., Zhang, Y., Xu, Z., Si, Z., Cheng, Y., Cheng, D., & Liu, G. (2022). Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F (ab) 2-Based SPECT/CT Imaging. Molecular Imaging, 2022.(IF 3.25)
Ren, H. N., Zhuo, T. X., Bai, S. J., Bai, Y., Sun, X. Y., Liu, R. D., ... & Wang, Z. Q. (2021). Proteomic analysis of hydrolytic proteases in excretory/secretory proteins from Trichinella spiralis intestinal infective larvae using zymography combined with shotgun LC-MS/MS approach. Acta Tropica, 216, 105825.(IF 3.112)
Liu, S., Li, Q., Liu, F., Cao, H., Liu, J., Shan, J., ... & Lin, J. (2021). Uncovering the mechanism of curcuma in the treatment of ulcerative colitis based on network pharmacology, molecular docking technology, and experiment verification. Evidence-Based Complementary and Alternative Medicine, 2021.(IF 2.629)
Wu, F., Song, L., Gong, Y., Wang, Y., Li, H., & Zhang, S. (2023). Antimicrobial Peptide Pt5‐1c Promotes Keratinocyte Migration and Proliferation via EGFR‐mediated Akt/MAPK/STAT3 pathways. ChemistrySelect, 8(2), e202203707.(IF 2.307)
Luo, Q., Qiu, L., Zhan, K., Zeng, L., Liao, S., Li, C., ... & Lv, L. (2023). Peroxisomal trans-2-enoyl-CoA inhibits proliferation, migration and invasion of hepatocellular carcinoma cells. Acta Histochemica, 125(2), 152002.(IF 2.147)
Copyright © 2023上海雅酶生物医药科技有限公司（Epizyme Biotech）
沪ICP备2021002422号
