本产品适用于Tris-甘氨酸电泳体系，采用上层胶和下层胶的预混配方，只需加入改良型促凝剂即可凝胶，灌入下层胶后，无需等待凝胶，直接灌入上层胶即可，简便快捷。所配的上层胶带有颜色(红色、蓝色或绿色)，点样孔清晰易辨，方便点样。三种颜色设计，可用于区分含不同样品的凝胶。本试剂盒灌制的凝胶也可用于非变性PAGE凝胶电泳。
本产品配套提供改良型促凝剂 ，其具有更好的稳定性和催化效能，配胶过程中无需额外添加TEMED。为方便操作，已开盖的改良型促凝剂可置于4℃保存至少三个月。
PG210（制备 6% 的PAGE胶）
PG211（制备 7.5% 的PAGE胶）
PG212（制备 10% 的PAGE胶）
PG213（制备 12.5% 的PAGE胶）
PG214（制备 15% 的PAGE胶）
125块(0.75 mm胶)
或
＞90块(1.00 mm胶)
或
＞60块(1.50 mm胶)
一步法灌胶
灌制下层胶后直接注入上层胶，无需液封操作简单快速
无需计算所需溶液量，无需稀释彩色上层胶
可制备红蓝绿三种颜色的上层胶，为点样和区分不同凝胶提供便利避免异味
无需使用TEMED，避免恶臭气味条带清晰
尤其小分子蛋白质条带比在传统凝胶中更清晰取等体积下层胶溶液和下层胶缓冲液，各2.0/2.7/4.0mL，混匀；
取等体积上层胶溶液和彩色上层胶缓冲液，各0.5/0.75/1.0mL，混匀；
注意：由于染料的特殊理化性质，使用前请摇匀。
向步骤1的混合溶液中加入40/60/80μL的改良型促凝剂，轻轻混匀，将混匀后的溶液注入制胶玻璃板中，使液面和短玻璃板上沿之间的距离比梳齿长0.5cm即可；
注意：①此溶液为过量，请勿全部注入;
②加入改良型促凝剂后，需轻柔混匀，防止过多氧气混入胶溶液，抑制凝胶聚合。
向步骤2的混合溶液中加入10/15/20μL的改良型促凝剂，轻轻混匀，无需等待下层胶凝固，即可将混匀后的溶液轻缓注入制胶玻璃板中，插入梳齿;
注意：①灌注上层胶溶液一定要轻缓，避免将上层胶溶液冲入下层胶;
②加入改良型促凝剂后，需轻柔混匀，防止过多氧气混入胶溶液，抑制凝胶聚合。
待胶凝固后(约15min)，拔去梳齿即可用于电泳。
注意：①请尽量使用新鲜配制的电泳缓冲液;
②胶凝固后上下层胶分界线平整度略弱于传统方法配的胶，但对后续电泳没有影响。
凝胶浓度选择参考
1. 本产品制备出的凝胶其上层胶对样品没有浓缩效应，与预制胶类似，但与传统PAGE胶相比，对蛋白条带分离效果更好， 小分子量蛋白(比如10kDa)也可以清晰地分离开，且蛋白条带更窄更锐利；
2. 不同浓度试剂盒各组分请勿混用，否则会影响制胶及电泳效果；
3. 改良型促凝剂的使用量仅作参考，实际用量可根据个人实验习惯和经验调整。加入较多量的促凝剂可加速凝胶，反之亦然；
4. 凝胶速度与温度有显著的正相关性。同等条件下，温度越高，凝胶速度越快，室温过高时建议适当减小改良型促凝剂的用量；相反，如果室温较低，可适当延长凝胶时间；
5. 本产品已加入适量TEMED的替代品，如需进一步加速凝胶，临配胶前可按需补充适量TEMED；
6. 在配胶之前，使胶溶液及缓冲液平衡到室温(如室温放置几分钟)，可有效避免凝胶中气泡的形成；
7. 推荐电泳条件为：150V，约60min(或200V，约45min)；
8. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
9. 本产品仅限科研使用。
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