本产品是雅酶生物最新研发的快速、高灵敏、低背景、无毒无污染的考马斯亮蓝染色液。可用于SDS-PAGE及非变性胶中蛋白质的快速染色。最短染色15 min即可观察到纳克级别的蛋白条带,且背景极低,纯水漂洗处理即能进一步降低背景,而无需有毒的脱色液脱色处理。
快速灵敏
最少仅需染色15min即可观察低至10ng的蛋白条带,适当延长染色时间可进一步提高灵敏度背景极低
染色结束后即可观察到清晰的蛋白条带,背景极低,使用纯水洗涤即可进一步降低背景染色,提高信噪比环保无毒
本品使用环保配方,无毒,无刺激性气味,保护实验人员健康电泳结束后,将凝胶置于纯水中漂洗数分钟去除杂质,以降低背景;
弃去纯水,加入适量本品,以覆盖凝胶为宜,室温下于水平摇床上染色10~15 min。如蛋白量较低,可适当延长染色时间,实际染色时间可根据条带显现程度决定。染色结束后,弃去染色液,加入纯水洗涤去除残留染液,即可观察结果;
如需进一步降低染色背景,可每隔20min更换纯水进行漂洗,重复4~5次即可得到背景极低的凝胶。此外,如将染色后的凝胶在纯水中浸泡过夜,可以达到更好的去背景效果,从而获得更清晰的蛋白条带。
1. 本试剂含挥发性物质,请注意密封以避免失效;
2. 如染色后蛋白条带不明显,则说明蛋白含量过低。建议延长染色时间或延长纯水洗涤时间,然后再进行观察。同时,点样时可加入阳性对照,以检测染色效果;
3. 由于SDS会干扰考马斯亮蓝与蛋白质的结合,可以先使用加热后的纯水漂洗凝胶,去除胶中残留的SDS再进行染色,效果更佳;
4. 为了您的安全和健康,请穿实验服并戴一次性手套操作;
5. 本产品仅限科研使用。
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