Omni-RapidTM快速蛋白定量试剂盒的原理与传统BCA蛋白定量法类似，但采用了一种不同于BCA(Bicin-choninic Acid)的全新特殊的螯合剂，从而实现了对蛋白质浓度进行快速、稳定、灵敏的测定。其原理是在碱性环境下蛋白质分子中的肽键能与Cu2+形成络合物，将Cu2+还原成Cu+，Cu+与螯合物结合，从而发生颜色反应。本试剂盒中的螯合剂可敏感特异地与Cu+结合，只需室温孵育5min即可形成稳定的橙黄色水溶性复合物，而传统BCA法则需在37℃下孵育30min才可完成颜色反应。该橙黄色的复合物在480nm处有强光吸收值，颜色的深浅与蛋白质浓度成正比，可根据吸收值的大小来测定蛋白质的含量。本试剂盒含有一系列浓度的蛋白质标准品溶液(BSA溶液),即取即用，无需稀释，方便快捷。
简单快速
室温5min完成显色反应方便快捷
提供即用型标准品，省去繁琐的稀释步骤准确性高
变异系数远小于考马斯亮蓝染色法线性范围宽
灵敏，检测范围：20~2,000μg/mL兼容性好
与大多数金属离子、螯合剂及去污剂兼容性较好配置显色工作液：
a. 计算显色工作液总量：
工作液总量 = (BSA标准品样本个数＋待测样本个数)×复孔数×每个样本显色工作液体积
举例：BSA标准品样本个数为8个，待测样本个数3个，复孔数3个。
显色工作液总量 = (8个BSA标准品样本＋3个待测样本)×3个复孔×200μL(每个样本工作液体积) = 6.6 mL
b. 根据计算出的所需显色工作液用量，将试剂A和试剂B按照50:1的体积比，配制显色工作液，充分混匀。
注意：
⑴试剂B刚加入试剂A时，会出现灰蓝色沉淀，但只需混匀几秒钟，沉淀就会消失，形成透亮的绿色溶液；
⑵建议工作液现用现配，在室温下，工作液会逐渐变为深绿色，但只要在1.5h内使用，对定量的准确性不会造成影响。
⑵由于加样可能存在误差，建议配制BCA工作液时，多配制1~2个孔。
定量检测：
① 分别取 即用型BSA标准品①~⑧ 各20μL加到 96孔板中( BSA标准品 使用前须充分溶解摇匀)
② 用1×PBS或0.9%生理盐水将样品适当稀释(可以多作几个梯度，如2倍、4倍、8倍稀释)，加20μL到96孔板的样品孔中；
③ 各孔加入200μL显色工作液，充分混匀，盖上96孔板盖，室温孵育5min，即可进行检测；
注意：由于颜色反应速度较快，须保证在20~30min之内完成读值。如果必须在30min后才能读值，可提前加入50μL 1 M HCl终止反应。
④ 用酶标仪测定每个样品及BSA标准品的A480，注意要减去空白对照( 标准品① ＋工作液)的A480。
⑤ 绘制标准曲线，计算样品中的蛋白浓度。
注意：数据处理时需要去除明显错误的值。待测样品浓度可以从标准曲线中查得, 实际浓度需要乘以样品的稀释倍数。如果是计算机绘制的曲线，可以从计算机给出的线性方程式计算出待测样品的浓度。
1. 本产品可以采用酶标仪(微孔检测法)或者分光光度计(试管检测法)测定蛋白浓度，如使用普通的分光光度计测定，需根据比色皿的最小检测体积，适当加大BCA工作液的用量使不小于最小检测体积，样品和标准品的用量可相应按比例放大也可不变。使用分光光度计测定蛋白浓度时，每个试剂盒可以测定的样品数量可能会显著减少；
2. 建议每次测定蛋白样品时，都须绘制标准曲线，以获得准确数据解；
3. 完成室温孵育5 min后，须在20~30 min内完成检测，否则会影响蛋白定量的准确度；
4. 如待测样品中含较多的干扰物质(具体见附表)，可采用其它蛋白定量产品；
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
6. 本产品仅限科研使用。
Zhang, S., Xia, S., Chen, L., Chen, Y., & Zhou, J. (2023). Covalent Organic Framework Nanobowls as Activatable Nanosensitizers for Tumor‐Specific and Ferroptosis‐Augmented Sonodynamic Therapy. Advanced Science, 2206009.(IF 17.521)
Wang, F., Wu, Q., Jia, G., Kong, L., Zuo, R., Feng, K., ... & Kang, Q. (2023). Black Phosphorus/MnO2 Nanocomposite Disrupting Bacterial Thermotolerance for Efficient Mild‐Temperature Photothermal Therapy. Advanced Science, 10(30), 2303911.(IF 15.1)
Liu, Y., Tang, S., Wang, X., Wang, X., Tang, X., Wu, Q., ... & Ding, J. (2023). A novel thermostable and salt-tolerant carboxylesterase involved in the initial aerobic degradation pathway for pyrethroids in Glycomyces salinus. Journal of Hazardous Materials, 451, 131128.(IF 14.224)
Dai, Z., Zhu, W., Hou, Y., Zhang, X., Ren, X., Lei, K., ... & Kuang, M. (2023). METTL5-mediated 18S rRNA m6A modification promotes oncogenic mRNA translation and intrahepatic cholangiocarcinoma progression. Molecular Therapy, 31(11), 3225-3242.(IF 12.4)
Liu, J., Peng, F., Cheng, H., Zhang, D., Zhang, Y., Wang, L., ... & Peng, C. (2023). Chronic cold environment regulates rheumatoid arthritis through modulation of gut microbiota-derived bile acids. Science of The Total Environment, 903, 166837.(IF 9.8)
Deng, S., Li, C., Chen, J., Cui, Z., Lei, T., Yang, H., & Chen, P. (2023). Effects of triclosan exposure on stem cells from human exfoliated deciduous teeth (SHED) fate. Science of The Total Environment, 905, 167053.(IF 9.8)
Yan, G., Li, L., Zhu, S., Wu, Y., Liu, Y., Zhu, L., ... & Wang, X. (2021). Single-cell transcriptomic analysis reveals the critical molecular pattern of UV-induced cutaneous squamous cell carcinoma. Cell death & disease, 13(1), 1-12.(IF 9.685)
Luo, L., Liu, M., Fan, Y., Zhang, J., Liu, L., Li, Y., Zhang, Q., Xie, H., Jiang, C., Wu, J., Xiao, X., & Wu, Y. (2022). Intermittent theta-burst stimulation improves motor function by inhibiting neuronal pyroptosis and regulating microglial polarization via TLR4/NFκB/NLRP3 signaling pathway in cerebral ischemic mice. Journal of Neuroinflammation, 19, 141.(IF 9.587)
Wang, Y., Niu, H., Li, L., Han, J., Liu, Z., Chu, M., ... & Zhao, J. (2023). Anti-CHAC1 exosomes for nose-to-brain delivery of miR-760-3p in cerebral ischemia/reperfusion injury mice inhibiting neuron ferroptosis. Journal of Nanobiotechnology, 21(1), 1-19.(IF 9.429)
Chen, Y., Li, D., Zhang, X., Ma, Q., Xu, Y., & Luo, Z. (2022). Azacytidine-induced hypomethylation delays senescence and coloration in harvested strawberries by stimulating antioxidant enzymes and modulating abscisate metabolism to minimize anthocyanin overproduction. Food Chemistry, 135189.(IF 9.231)
Wang, Z., Zhang, T., Jia, F., Ge, C., He, Y., Tian, Y., ... & Jiang, Y. (2023). Homologous Sequential Immunization Using Salmonella Oral Administration Followed by an Intranasal Boost with Ferritin-Based Nanoparticles Enhanced the Humoral Immune Response against H1N1 Influenza Virus. Microbiology Spectrum, e00102-23.(IF 9.043)
Ding, J., Liu, Y., Gao, Y., Zhang, C., Wang, Y., Xu, B., ... & Huang, Z. (2022). Biodegradation of λ-cyhalothrin through cell surface display of bacterial carboxylesterase. Chemosphere, 289, 133130.(IF 8.943)
Lu, A. X., Lin, Y., Li, J., Liu, J. X., Yan, C. H., & Zhang, L. (2023). Effects of food-borne docosahexaenoic acid supplementation on bone lead mobilisation, mitochondrial function and serum metabolomics in pre-pregnancy lead-exposed lactating rats. Environmental Pollution, 337, 122613.(IF 8.9)
Li, Z., Teng, M., Jiang, Y., Zhang, L., Luo, X., Liao, Y., & Yang, B. (2022). YTHDF1 Negatively Regulates Treponema pallidum-Induced Inflammation in THP-1 Macrophages by Promoting SOCS3 Translation in an m6A-Dependent Manner. Frontiers in Immunology, 13.(IF 8.786)
Hua, T., Wang, H., Fan, X., An, N., Li, J., Song, H., ... & Yuan, H. (2022). BRD4 inhibition attenuates inflammatory pain by ameliorating NLRP3 inflammasome-induced pyroptosis. Frontiers in immunology, 13: 837977.(IF 8.786)
Li, S., Wang, R., Huang, L., Jiang, Y., **ng, F., Duan, W., ... & **e, H. (2023). Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine‐derived stem cells. Cell Proliferation, e13542.(IF 8.5)
Zhang, X., Zhang, S., Qi, J., Zhao, F., Lu, Y., Li, S., ... & Tan, J. (2023). PDGFBB improved the biological function of menstrual blood-derived stromal cells and the anti-fibrotic properties of exosomes. Stem Cell Research & Therapy, 14(1), 1-14.(IF 8.079)
Zhou, Y., Zhao, X., Hu, W., Ruan, F., He, C., Huang, J., & Zuo, Z. (2021). Acute and subacute oral toxicity of propylene glycol enantiomers in mice and the underlying nephrotoxic mechanism. Environmental Pollution, 290, 118050.(IF 8.071)
Li, S., Wang, Y., Jiang, H., Bai, Y., Chen, T., Chen, M., ... & Chen, Y. (2023). Display of CCL21 on cancer cell membrane through genetic modification using a pH low insertion peptide. International Journal of Biological Macromolecules, 240, 124324.(IF 8.025)
Lai, J., Li, Y., Ran, M., Huang, Q., Huang, F., Zhu, L., ... & Wu, J. (2023). Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling. Biomedicine & Pharmacotherapy, 163, 114811.(IF 7.419)
Liu, Z., Nan, P., Gong, Y., Tian, L., Zheng, Y., & Wu, Z. (2023). Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy. Biomedicine & Pharmacotherapy, 164, 114897.(IF 7.419)
Tan, X., Gong, L., Li, X., Zhang, X., Sun, J., Luo, X., ... & Han, S. (2021). Promethazine inhibits proliferation and promotes apoptosis in colorectal cancer cells by suppressing the PI3K/AKT pathway. Biomedicine & Pharmacotherapy, 143, 112174.(IF 7.419)
Fang, J., Wu, H., Zhang, J., Mao, S., Shi, H., Yu, D., ... & Shi, H. (2022). A reduced form of nicotinamide riboside protects the cochlea against aminoglycoside-induced ototoxicity by SIRT1 activation. Biomedicine & Pharmacotherapy, 150, 113071.(IF 7.419)
Wang, C. X., Wang, T. T., Zhang, K. D., Li, M. Y., Shen, Q. C., Lu, S. Y., & Zhang, J. (2022). Pan-KRAS inhibitors suppress proliferation through feedback regulation in pancreatic ductal adenocarcinoma. Acta Pharmacologica Sinica, 1-13.(IF 7.169)
Xu, X., Chang, J., Wang, P., Liu, C., Liu, M., Zhou, T., ... & Yan, G. (2023). Combination of glycyrrhizic acid and compound probiotics alleviates deoxynivalenol-induced damage to weaned piglets. Ecotoxicology and Environmental Safety, 256, 114901.(IF 7.129)
Wang, F., Kong, L., Wang, W., Shi, L., Wang, M., Chai, Y., ... & Kang, Q. (2021). Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis. Stem cell research & therapy, 12(1), 1-15.(IF 6.832)
Wang F, Wang W, Kong L, et al. Accelerated bone regeneration by Adrenomedullin 2 through improving the coupling of osteogenesis and angiogenesis via β-catenin signaling[J]. Frontiers in Cell and Developmental Biology, 2021, 9.(IF 6.684)
Gu, K., Wang, T., Peng, L., & Zhao, Y. (2022). FIP-fve Stimulates Cell Proliferation and Enhances IL-2 Release by Activating MAP2K3/p38α (MAPK14) Signaling Pathway in Jurkat E6-1 Cells. Frontiers in Nutrition, 9.(IF 6.59)
Wang, F., Qian, H., Kong, L., Wang, W., Wang, X., Xu, Z., ... & Kang, Q. (2021). Accelerated bone regeneration by astragaloside IV through stimulating the coupling of osteogenesis and angiogenesis. International Journal of Biological Sciences, 17(7), 1821.(IF 6.58)
Chen Q, Yin H, Pu N, et al. Chemokine C‐C motif ligand 21 synergized with programmed death‐ligand 1 blockade restrains tumor growth[J]. Cancer science, 2021, 112(11): 4457-4469.(IF 6.518)
Liu, Y., Wang, X., Nong, S., Bai, Z., Han, N., Wu, Q., Huang, Z., & Ding, J. (2022). Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation. Microbial cell factories, 21(1), 97.(IF 6.352)
Zhao, Y., Jiang, Q., Guo, L., Fan, D., Wang, M., & Zhao, Y. (2023). Apigenin and its octoic acid diester attenuated glycidol-induced autophagic-dependent apoptosis via inhibiting the ERK/JNK/p38 signaling pathways in human umbilical vein endothelial cells (HUVECs). Current Research in Food Science, 100447.(IF 6.269)
Liu, C., Zhang, L., Liu, Y., Zhao, Q., Pan, Y., & Zhang, Y. (2021). Value of pyruvate carboxylase in thyroid fine-needle aspiration wash-out fluid for predicting papillary thyroid cancer lymph node metastasis. Frontiers in oncology, 11, 1625.(IF 6.244)
Shao, D., Gao, Z., Zhao, Y., Fan, M., Zhao, X., Wei, Q., ... & Ma, B. (2023). Sulforaphane Suppresses H2O2-Induced Oxidative Stress and Apoptosis via the Activation of AMPK/NFE2L2 Signaling Pathway in Goat Mammary Epithelial Cells. International Journal of Molecular Sciences, 24(2), 1070.(IF 6.208)
Jiang, Y., Li, Z., Ma, Q., Dong, W., Yao, Q., & Yu, D. (2023). Aucubin protects mouse cochlear hair cells from cisplatin-induced ototoxicity via activation of the PI3K/AKT/STAT3 pathway. Biochemical Pharmacology, 115440.(IF 6.1)
Su, J., Wang, X., Yin, C., Li, Y., Wu, H., Yu, W., & Han, F. (2021). Identification and Biochemical Characterization of a Surfactant-Tolerant Chondroitinase VhChlABC from Vibrio hyugaensis LWW-1. Marine Drugs, 19(7), 399.(IF 6.085)
Cong, P., Wu, T., Huang, X., Liang, H., Gao, X., Tian, L., ... & Xiong, L. (2021). Identification of the Role and Clinical Prognostic Value of Target Genes of m6A RNA Methylation Regulators in Glioma. Frontiers in Cell and Developmental Biology, 9, 709022-709022.(IF 6.081)
Zhou, S., Wu, H., Ning, W., Wu, X., Xu, X., Ma, Y., Li, X., Hu, J., Wang, C., & Wang, J. (2021). Ivermectin has New Application in Inhibiting Colorectal Cancer Cell Growth. Frontiers in pharmacology, 12, 717529.(IF 5.988)
Copyright © 2023上海雅酶生物医药科技有限公司（Epizyme Biotech）
沪ICP备2021002422号
