本品是一种广谱磷酸酶抑制剂，高效抑制Serine/Threonine Phosphatase，Tyrosine Phosphatase， Acid Phosphatase，Alkaline phosphatase 等细胞内所含的磷酸酶。
高效全面
多达8种的高效抑制成分，提供比同类产品(一般含3~4种抑制剂)更为全面的磷酸酶抑制能力含Sodium fluoride，Sodium orthovanadate，Sodium molybdate，Sodium tartrate，Imidazole，Sodium pyrophosphate decahydrate，β-Glycerophosphate，Cantharidin 等8种高效磷酸酶抑制剂。
1. 本品用于各种蛋白质含量或其活性分析实验(Western Blot，Co-IP，pull-down，IF，IHC 等)，抑制磷酸酶的活性；
2. 将本品以1:100(体积比)的稀释比加入到目标溶液中(如细胞裂解液，蛋白提取液等)。
1. 当与蛋白酶抑制剂联用时，请不要使用含有EDTA的蛋白酶抑制剂，因为EDTA会严重影响 磷酸酶抑制剂混合液中某些成分的抑制效果。事实上磷酸酶抑制剂混合液中已含有多种螯合剂成分；
2. 本品为高浓度溶液，长期存放可能会出现微量晶体状沉淀，此为正常现象，不影响实验结果；
3. 不同来源的样品中磷酸酶含量各不相同，1:100为推荐的常规剂量。实际使用中，如发现磷酸酶含量较高，可适当提高磷酸酶抑制剂混合液的使用浓度；
4. 为了您的安全和健康，请穿实验服并戴一次性手套操作。所有抑制剂对人体均有毒，请勿直接与皮肤接触。如不小心接触皮肤，请立即用大量清水冲洗。如不慎吞食，应立即就医处理；
5. 本产品仅限科研使用。
Zhang, C., Teng, Y., Li, F., Ho, W., Bai, X., Xu, X., & Zhang, X. Q. (2023). Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells. ACS nano.(IF 17.1)
Wang, F., Li, Z., Zhou, J., Wang, G., Zhang, W., Xu, J., & Liang, A. (2021). SIRT1 regulates the phosphorylation and degradation of P27 by deacetylating CDK2 to promote T-cell acute lymphoblastic leukemia progression. Journal of Experimental & Clinical Cancer Research, 40(1), 1-16.(IF 12.658)
Li, H., Chang, L., Wu, J., Huang, J., Guan, W., Bates, L. E., ... & Silva, J. C. (2023). In vitro generation of mouse morula-like cells. Developmental Cell, 58(22), 2510-2527.(IF 11.8)
Huang, Y., Chen, S., Wu, S., Dong, G., & Sheng, C. (2020). Evodiamine-inspired dual inhibitors of histone deacetylase 1 (HDAC1) and topoisomerase 2 (TOP2) with potent antitumor activity. Acta Pharmaceutica Sinica B, 10(7), 1294-1308.(IF 11.413)
Li, X., Wang, Y., Zhai, Z., Mao, Q., Chen, D., **ao, L., ... & Chen, T. (2023). Predicting response to immunotherapy in gastric cancer via assessing perineural invasion-mediated inflammation in tumor microenvironment. Journal of Experimental & Clinical Cancer Research, 42(1), 206.(IF 11.3)
Li, J., Han, X., Sun, M., Li, W., Yang, G., Chen, H., ... & Wang, H. (2023). Caspase-9 inhibition triggers Hsp90-based chemotherapy-mediated tumor intrinsic innate sensing and enhances antitumor immunity. Journal for Immunotherapy of Cancer, 11(12).(IF 10.9)
Liu, J., Huang, Z., Yin, S., Zhou, X., Jiang, Y., & Shao, L. (2023). The lysosome-mitochondrion crosstalk engaged in silver nanoparticles-disturbed mitochondrial homeostasis. Science of The Total Environment, 889, 164078.(IF 10.753)
Chen, S., Bi, K., Liang, H., Wu, Z., Huang, M., Chen, X., ... & Sheng, C. (2023). PROTAC derivatization of natural products for target identification and drug discovery: Design of evodiamine-based PROTACs as novel REXO4 degraders. Journal of Advanced Research.(IF 10.7)
Zhao, S., **u, G., Wang, J., Wen, Y., Lu, J., Wu, B., ... & Xu, J. (2023). Engineering exosomes derived from subcutaneous fat MSCs specially promote cartilage repair as miR-199a-3p delivery vehicles in Osteoarthritis. Journal of Nanobiotechnology, 21(1), 341.(IF 10.2)
Li, J., Han, X., Gao, S., Yan, Y., Li, X., & Wang, H. (2023). Tumor microenvironment-responsive DNA-based nanomedicine triggers innate sensing for enhanced immunotherapy. Journal of Nanobiotechnology, 21(1), 382.(IF 10.2)
Jiang, Z. X., Wang, Y. N., Li, Z. Y., Dai, Z. H., He, Y., Chu, K., ... & Li, W. (2021). The m6A mRNA demethylase FTO in granulosa cells retards FOS-dependent ovarian aging. Cell death & disease, 12(8), 1-10.(IF 9.685)
Ding, C., Yang, C., Cheng, T., Wang, X., Wang, Q., He, R., ... & Zhang, X. (2021). Macrophage-biomimetic porous Se@ SiO2 nanocomposites for dual modal immunotherapy against inflammatory osteolysis. Journal of nanobiotechnology, 19(1), 1-16.(IF 9.429)
Zou, H., Luo, J., Guo, Y., Tong, T., Liu, Y., Chen, Y., ... & Li, P. (2023). Tyrosine kinase SRC-induced YAP1-KLF5 module regulates cancer stemness and metastasis in triple-negative breast cancer. Cellular and Molecular Life Sciences, 80(2), 1-17.(IF 9.207)
Zhu, Y., Tang, X., Xu, Y., Wu, S., Liu, W., Geng, L., ... & Sun, L. (2022). RNASE2 Mediates Age-Associated B Cell Expansion Through Monocyte Derived IL-10 in Patients With Systemic Lupus Erythematosus. Frontiers in Immunology, 13, 752189-752189.(IF 8.786)
Hua, T., Wang, H., Fan, X., An, N., Li, J., Song, H., ... & Yuan, H. (2022). BRD4 inhibition attenuates inflammatory pain by ameliorating NLRP3 inflammasome-induced pyroptosis. Frontiers in immunology, 13: 837977.(IF 8.786)
Xing, Y., Peng, K., Yi, Q., Yu, D., Shi, H., Yang, G., & Yin, S. (2023). TMEM30A is essential for hair cell polarity maintenance in postnatal mouse cochlea. Cellular & Molecular Biology Letters, 28(1), 1-24.(IF 8.702)
Chen, X. X., Tao, T., Liu, X. Z., Wu, W., Wang, J. W., Yue, T. T., ... & Li, W. (2023). P38-DAPK1 axis regulated LC3-associated phagocytosis (LAP) of microglia in an in vitro subarachnoid hemorrhage model. Cell Communication and Signaling, 21(1), 1-15.(IF 8.4)
Chen, S., Li, B., Chen, L., & Jiang, H. (2023). Uncovering the mechanism of resveratrol in the treatment of diabetic kidney disease based on network pharmacology, molecular docking, and experimental validation. Journal of Translational Medicine, 21(1), 1-14.(IF 7.4)
Liu, X., Chen, C., Han, D., Zhou, W., Cui, Y., Tang, X., ... & Gao, Y. (2022). SLC7A11/GPX4 inactivation-mediated ferroptosis contributes to the pathogenesis of triptolide-induced cardiotoxicity. Oxidative medicine and cellular longevity, 2022.(IF 7.31)
Xu, Z., Shi, L., Li, D., Wu, Q., Zhang, Y., Gao, M., ... & Cui, L. (2022). Real ambient particulate matter-induced lipid metabolism disorder: Roles of peroxisome proliferators-activated receptor alpha. Ecotoxicology and Environmental Safety, 231, 113173.(IF 7.129)
Zhao, Y., Jiang, Q., Guo, L., Fan, D., Wang, M., & Zhao, Y. (2023). Apigenin and its octoic acid diester attenuated glycidol-induced autophagic-dependent apoptosis via inhibiting the ERK/JNK/p38 signaling pathways in human umbilical vein endothelial cells (HUVECs). Current Research in Food Science, 100447.(IF 6.269)
Cheng, J., Li, Y., Wang, X., Dong, G., & Sheng, C. (2020). Discovery of Novel PDEδ Degraders for the Treatment of KRAS Mutant Colorectal Cancer. Journal of Medicinal Chemistry, 63(14), 7892-7905.(IF 6.205)
Jiang, Y., Li, Z., Ma, Q., Dong, W., Yao, Q., & Yu, D. (2023). Aucubin protects mouse cochlear hair cells from cisplatin-induced ototoxicity via activation of the PI3K/AKT/STAT3 pathway. Biochemical Pharmacology, 115440.(IF 6.1)
Wu, P., Lin, B., Huang, S., Meng, J., Zhang, F., Zhou, M., Hei, X., Ke, Y., Yang, H., & Huang, D. (2022). IL-11 Is Elevated and Drives the Profibrotic Phenotype Transition of Orbital Fibroblasts in Thyroid-Associated Ophthalmopathy. Frontiers in endocrinology, 13, 846106.(IF 6.055)
Zhang, P., Lai, X., Zhu, M. H., Long, M., Liu, X. L., Wang, Z. X., ... & Zhao, M. (2021). Saikosaponin A, a Triterpene Saponin, Suppresses Angiogenesis and Tumor Growth by Blocking VEGFR2-Mediated Signaling Pathway. Frontiers in pharmacology, 12.(IF 5.988)
Gao, M., Ma, Y., Luo, J., Li, D., Jiang, M., Jiang, Q., ... & Cui, L. (2021). The role of Nrf2 in the PM-induced vascular injury under real ambient particulate matter exposure in C57/B6 mice. Frontiers in pharmacology, 12, 618023.(IF 5.81)
Zhao, S., Wang, J., Wen, Y., Wu, B., Yang, D., Wang, G., ... & Xu, J. (2023). ADSCs increase the autophagy of chondrocytes through decreasing miR-7-5p in Osteoarthritis rats by targeting ATG4A. International Immunopharmacology, 120, 110390.(IF 5.714)
Yu, J., Jiang, Q., Liu, N., Fan, D., Wang, M., & Zhao, Y. (2022). Apigenin and apigenin-7, 4′-O-dioctanoate protect against acrolein-aggravated inflammation via inhibiting the activation of NLRP3 inflammasome and HMGB1/MYD88/NF-κB signaling pathway in Human umbilical vein endothelial cells (HUVEC). Food and Chemical Toxicology, 168, 113400.(IF 5.572)
Zheng, R., Pan, Y., Liu, X., Liu, F., Li, A., Zheng, D., & Luo, Y. (2023). Comprehensive analysis of REST corepressors (RCORs) in pan-cancer. Frontiers in Cell and Developmental Biology, 11, 1162344.(IF 5.5)
Hua, H., Pan, C., Chen, X., Jing, M., Xie, J., Gao, Y., ... & Li, P. (2023). Probiotic lactic acid bacteria alleviate pediatric IBD and remodel gut microbiota by modulating macrophage polarization and suppressing epithelial apoptosis. Frontiers in Microbiology, 14, 1168924.(IF 5.2)
He, W., Cheng, Z., Huo, Z., Lin, B., Wang, X., Sun, Y., ... & Xiao, H. (2023). STRA6 promotes thyroid carcinoma progression via activation of the ILK/AKT/mTOR axis in cells and female nude mice. Endocrinology, bqac215-bqac215.(IF 5.051)
Pan, C., Chen, L., Wu, R., Shan, H., Zhou, Z., Lin, Y., ... & Wu, C. (2018). Lithium-containing biomaterials inhibit osteoclastogenesis of macrophages in vitro and osteolysis in vivo. Journal of Materials Chemistry B, 6(48), 8115-8126.(IF 5.047)
Ma, Y., Zheng, K., Pang, Y., Xiang, F., Gao, J., Zhang, C., & Du, D. (2022). Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs. Journal of Orthopaedic Translation, 32, 59-68.(IF 4.889)
Zhu, Y., Tao, Z., Chen, Y., Lin, S., Zhu, M., Ji, W., ... & Hu, X. (2022). Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT. Breast Cancer Research and Treatment, 193(1), 65-81.(IF 4.624)
Cai, Y., Li, X., Pan, Z., Zhu, Y., Tuo, J., Meng, Q., ... & Pan, Y. (2020). Anthocyanin ameliorates hypoxia and ischemia induced inflammation and apoptosis by increasing autophagic flux in SH-SY5Y cells. European Journal of Pharmacology, 883, 173360.(IF 4.432)
Li, K., Gong, Q., Lu, B., Huang, K., Tong, Y., Mutsvene, T. E., ... & Hu, L. (2023). Anti-inflammatory and antioxidative effects of gallic acid on experimental dry eye: in vitro and in vivo studies. Eye and Vision, 10(1), 17.(IF 4.427)
Cui, L., Shi, L., Li, D., Li, X., Su, X., Chen, L., ... & Chen, C. (2020). Real-ambient particulate matter exposure-induced cardiotoxicity in C57/B6 mice. Frontiers in Pharmacology, 11, 199.(IF 4.225)
He, Z., Liu, X., Liu, X., Cui, L., Yuan, Y., Zhang, H., ... & Yu, Z. (2021). The role of MEG3 in the proliferation of palatal mesenchymal cells is related to the TGFβ/Smad pathway in TCDD inducing cleft palate. Toxicology and Applied Pharmacology, 419, 115517.(IF 4.219)
Gao, Y., Peng, Q., Li, S., Zheng, K., Gong, Y., Xue, Y., ... & Shi, X. (2023). YAP1 suppression inhibits autophagy and improves the efficacy of anti-PD-1 immunotherapy in hepatocellular carcinoma. Experimental Cell Research, 113486.(IF 4.145)
Yu, X., Zhu, D., Luo, B., Kou, W., Cheng, Y., & Zhu, Y. (2022). IFNγ enhances ferroptosis by increasing JAK-STAT pathway activation to suppress SLCA711 expression in adrenocortical carcinoma. Oncology reports, 47(5), 1-12.(IF 4.136)
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