本品是一种广谱蛋白酶抑制剂,全面抑制Serine Proteases,Cysteine Proteases,Aspartic Acid-proteases,Aminopeptidases和Metalloproteases等细胞内所含的蛋白酶。
高效全面
多达9种的高效抑制成分,提供比同类产品(一般含6种抑制剂)更为全面的蛋白保护作用含AEBSF,Aprotinin,Bestatin,E-64,Leupeptin,Pepstatin A,Phosphoramidon,Benzamidine HCl,1,10-phenanthroline等9种高效蛋白酶抑制剂。
1. 本品可用于各种蛋白质含量或其活性分析实验(Western Blot,Co-IP,pull-down,IF,IHC 等),保护蛋白质免受蛋白酶的降解;
2. 将本品以1:100(体积比)的稀释比加入到目标溶液中(如细胞裂解液,蛋白提取液等)。
1. 本品不含EDTA,如需使用时,需另行添加。当与磷酸酶抑制剂混合液(GRF102)联用时,建议不加EDTA,EDTA会严重影响磷酸酶抑制剂混合液中某些成分的抑制效果;
2. 本品为高浓度溶液,长期存放可能会出现微量晶体状沉淀,此为正常现象,不影响实验结果;
3. 不同来源的样品中蛋白酶含量各不相同,1:100为推荐的常规剂量。实际使用中,如发现蛋白酶含量较高,可适当提高蛋白酶抑制剂的使用浓度;
4. 为了您的安全和健康,请穿实验服并戴一次性手套操作;
5. 本产品仅限科研使用。
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